cfx96 real time system c1000 (Bio-Rad)
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cfx96 real time system c1000
Cfx96 Real Time System C1000, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15056 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx96+real+time+system+c1000/CFX96+and+CFX384+Real-Time+PCR+Detection+Systems+Firmware+Update/pmc12914680-144-61-65
Average 99 stars, based on 15056 article reviews
Cfx96 Real Time System C1000, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15056 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx96+real+time+system+c1000/CFX96+and+CFX384+Real-Time+PCR+Detection+Systems+Firmware+Update/pmc12914680-144-61-65
Average 99 stars, based on 15056 article reviews
cfx96 real time system c1000 - by Bioz Stars,
2026-09
99/100 stars
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Synthesized:Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach Article Snippet: The quantity and integrity of the mRNA were assessed using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and only samples with the required quality (260/280 and 260/230 ratio values of around 2) were used in the following step. .. The cDNA was synthesized from 500 ng RNA and performed using the NZY First-Strand cDNA Synthesis Kit, according to the manufacturer’s protocol (NZYTech, Lisbon, Portugal) in a Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach. Article Snippet: The quantity and integrity of the mRNA were assessed using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and only samples with the required quality (260/280 and 260/230 ratio values of around 2) were used in the following step. .. The cDNA was synthesized from 500 ng RNA and performed using the NZY FirstStrand cDNA Synthesis Kit, according to the manufacturer’s protocol (NZYTech, Lisbon, Portugal) in a cDNA Synthesis:Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach Article Snippet: The quantity and integrity of the mRNA were assessed using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and only samples with the required quality (260/280 and 260/230 ratio values of around 2) were used in the following step. .. The cDNA was synthesized from 500 ng RNA and performed using the NZY First-Strand cDNA Synthesis Kit, according to the manufacturer’s protocol (NZYTech, Lisbon, Portugal) in a Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach. Article Snippet: The quantity and integrity of the mRNA were assessed using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and only samples with the required quality (260/280 and 260/230 ratio values of around 2) were used in the following step. .. The cDNA was synthesized from 500 ng RNA and performed using the NZY FirstStrand cDNA Synthesis Kit, according to the manufacturer’s protocol (NZYTech, Lisbon, Portugal) in a Article Title: SET-M33 loaded biosynthesized cellulose as effective protection against S. aureus biofilm formation Article Snippet: The concentration and purity of the RNA was then checked using a quantification spectrophotometer (DeNovix®, USA). .. The SensiFASTTM cDNA synthesis kit (meridian BIOSCIENCE) was used to synthesize cDNA from 200 ng of total RNA. qPCR reactions were performed using 2.5 μL cDNA, 12.5 μL SYBR green, 0.5 μL MgCl 2 (SensiMixTM), and 0.75 μL of forward and reverse primers ( ) at a concentration of 10 μM in clear hardshell PCR plates (Bio-Rad Inc, USA) in a Reverse Transcription Polymerase Chain Reaction:Article Title: QTL mapping and identification of candidate genes for cold tolerance at the germination stage in wild rice. Article Snippet: Background Cold damage stress significantly affects rice growth (germination and seedling) and causes serious losses in yield in temperate and high-altitude areas around the globe.. Objective This study aimed to explore the cold tolerance (CT) locus of rice and create new cold-tolerant germplasm.. We constructed a chromosome segment substitution line (CSSL) with strong CT and fine mapped quantitative trait loci (QTLs) associated with CT by performing the whole-genome resequencing of CSSL with phenotypes under cold treatment. Real-time Polymerase Chain Reaction:Article Title: QTL mapping and identification of candidate genes for cold tolerance at the germination stage in wild rice. Article Snippet: Background Cold damage stress significantly affects rice growth (germination and seedling) and causes serious losses in yield in temperate and high-altitude areas around the globe.. Objective This study aimed to explore the cold tolerance (CT) locus of rice and create new cold-tolerant germplasm.. We constructed a chromosome segment substitution line (CSSL) with strong CT and fine mapped quantitative trait loci (QTLs) associated with CT by performing the whole-genome resequencing of CSSL with phenotypes under cold treatment. Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach. Article Snippet: .. The gene expression of aquaporins was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems, ThermoFisher Scientific, Waltham, MA, USA), and the following specific TaqMan pre-designed gene expression primers: AQP0 (Hs0085175_m1), AQP1 (Hs01028916_m1), AQP2 (Hs00166640_m1), AQP3 (Hs01105469_g1), AQP4 (Hs00242342_m1), AQP5 (Hs00387048_m1), AQP6 (Hs00155808_m1), AQP7 (Hs00357359_m1), AQP8 (Hs01086280_g1), AQP9 (Hs00175573_m1), AQP10 (Hs00369738_m1), AQP11 (Hs005426181_m1), and AQP12 (Hs01651303_m1) (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA). β-ACT (Hs99999903_m1) Biomolecules 2025, 15, 488 6 of 21 and HPRT-1 were used as housekeeping genes in cell cultures and tissues. qPCR reactions were carried out using a Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach Article Snippet: .. The gene expression of aquaporins was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems, ThermoFisher Scientific, Waltham, MA, USA), and the following specific TaqMan pre-designed gene expression primers: AQP0 (Hs0085175_m1), AQP1 (Hs01028916_m1), AQP2 (Hs00166640_m1), AQP3 (Hs01105469_g1), AQP4 (Hs00242342_m1), AQP5 (Hs00387048_m1), AQP6 (Hs00155808_m1), AQP7 (Hs00357359_m1), AQP8 (Hs01086280_g1), AQP9 (Hs00175573_m1), AQP10 (Hs00369738_m1), AQP11 (Hs005426181_m1), and AQP12 (Hs01651303_m1) (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA). β-ACT (Hs99999903_m1) and HPRT-1 were used as housekeeping genes in cell cultures and tissues. qPCR reactions were carried out using a Article Title: Aquaporin-1, aquaporin-3 and aquaporin-5 differentially modulate cell biophysical and biomechanical properties, impacting cell stiffness and cell-cell adhesion. Article Snippet: RNA was quantified using a Nanodrop 2000c spectrophotometer (ThermoFisher Scientific), and 1 μg of good quality RNA (260/280 nm and 260/230 nm ratios of 2) was used to synthesize cDNA with a first-strand cDNA synthesis kit (NZYtech, Lisbon, Portugal), according to the manufacturer’s instructions. .. Transcript levels of human AQP1, AQP3, and AQP5, and the housekeeping gene hypoxanthine phosphoribosyltransferase 1 (HPRT-1) were quantified through qPCR performed in a Article Title: SET-M33 loaded biosynthesized cellulose as effective protection against S. aureus biofilm formation Article Snippet: The concentration and purity of the RNA was then checked using a quantification spectrophotometer (DeNovix®, USA). .. The SensiFASTTM cDNA synthesis kit (meridian BIOSCIENCE) was used to synthesize cDNA from 200 ng of total RNA. qPCR reactions were performed using 2.5 μL cDNA, 12.5 μL SYBR green, 0.5 μL MgCl 2 (SensiMixTM), and 0.75 μL of forward and reverse primers ( ) at a concentration of 10 μM in clear hardshell PCR plates (Bio-Rad Inc, USA) in a Article Title: Blockage of aquaporin‐3 peroxiporin activity by organogold compounds affects melanoma cell adhesion, proliferation and migration Article Snippet: RNA quantity and quality were assessed using aNanodrop 2000c spectrophotometer and 1μg of good quality RNA (260/280 and 260/230 ratio of 2) was used to synthesize cDNA using the first-strand cDNA synthesis kit (NZYtech, Lisbon, Portugal) according to the manufacturer’s protocol. .. Real-time PCR reactions were carried out using a Quantitative RT-PCR:Article Title: QTL mapping and identification of candidate genes for cold tolerance at the germination stage in wild rice. Article Snippet: Background Cold damage stress significantly affects rice growth (germination and seedling) and causes serious losses in yield in temperate and high-altitude areas around the globe.. Objective This study aimed to explore the cold tolerance (CT) locus of rice and create new cold-tolerant germplasm.. We constructed a chromosome segment substitution line (CSSL) with strong CT and fine mapped quantitative trait loci (QTLs) associated with CT by performing the whole-genome resequencing of CSSL with phenotypes under cold treatment. Gene Expression:Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach. Article Snippet: .. The gene expression of aquaporins was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems, ThermoFisher Scientific, Waltham, MA, USA), and the following specific TaqMan pre-designed gene expression primers: AQP0 (Hs0085175_m1), AQP1 (Hs01028916_m1), AQP2 (Hs00166640_m1), AQP3 (Hs01105469_g1), AQP4 (Hs00242342_m1), AQP5 (Hs00387048_m1), AQP6 (Hs00155808_m1), AQP7 (Hs00357359_m1), AQP8 (Hs01086280_g1), AQP9 (Hs00175573_m1), AQP10 (Hs00369738_m1), AQP11 (Hs005426181_m1), and AQP12 (Hs01651303_m1) (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA). β-ACT (Hs99999903_m1) Biomolecules 2025, 15, 488 6 of 21 and HPRT-1 were used as housekeeping genes in cell cultures and tissues. qPCR reactions were carried out using a Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach Article Snippet: .. The gene expression of aquaporins was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems, ThermoFisher Scientific, Waltham, MA, USA), and the following specific TaqMan pre-designed gene expression primers: AQP0 (Hs0085175_m1), AQP1 (Hs01028916_m1), AQP2 (Hs00166640_m1), AQP3 (Hs01105469_g1), AQP4 (Hs00242342_m1), AQP5 (Hs00387048_m1), AQP6 (Hs00155808_m1), AQP7 (Hs00357359_m1), AQP8 (Hs01086280_g1), AQP9 (Hs00175573_m1), AQP10 (Hs00369738_m1), AQP11 (Hs005426181_m1), and AQP12 (Hs01651303_m1) (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA). β-ACT (Hs99999903_m1) and HPRT-1 were used as housekeeping genes in cell cultures and tissues. qPCR reactions were carried out using a Article Title: Aquaporin-1, aquaporin-3 and aquaporin-5 differentially modulate cell biophysical and biomechanical properties, impacting cell stiffness and cell-cell adhesion. Article Snippet: RNA was quantified using a Nanodrop 2000c spectrophotometer (ThermoFisher Scientific), and 1 μg of good quality RNA (260/280 nm and 260/230 nm ratios of 2) was used to synthesize cDNA with a first-strand cDNA synthesis kit (NZYtech, Lisbon, Portugal), according to the manufacturer’s instructions. .. Transcript levels of human AQP1, AQP3, and AQP5, and the housekeeping gene hypoxanthine phosphoribosyltransferase 1 (HPRT-1) were quantified through qPCR performed in a Article Title: Blockage of aquaporin‐3 peroxiporin activity by organogold compounds affects melanoma cell adhesion, proliferation and migration Article Snippet: RNA quantity and quality were assessed using aNanodrop 2000c spectrophotometer and 1μg of good quality RNA (260/280 and 260/230 ratio of 2) was used to synthesize cDNA using the first-strand cDNA synthesis kit (NZYtech, Lisbon, Portugal) according to the manufacturer’s protocol. .. Real-time PCR reactions were carried out using a Polymerase Chain Reaction:Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach. Article Snippet: .. The gene expression of aquaporins was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems, ThermoFisher Scientific, Waltham, MA, USA), and the following specific TaqMan pre-designed gene expression primers: AQP0 (Hs0085175_m1), AQP1 (Hs01028916_m1), AQP2 (Hs00166640_m1), AQP3 (Hs01105469_g1), AQP4 (Hs00242342_m1), AQP5 (Hs00387048_m1), AQP6 (Hs00155808_m1), AQP7 (Hs00357359_m1), AQP8 (Hs01086280_g1), AQP9 (Hs00175573_m1), AQP10 (Hs00369738_m1), AQP11 (Hs005426181_m1), and AQP12 (Hs01651303_m1) (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA). β-ACT (Hs99999903_m1) Biomolecules 2025, 15, 488 6 of 21 and HPRT-1 were used as housekeeping genes in cell cultures and tissues. qPCR reactions were carried out using a Article Title: The Association of Aquaporins with MAPK Signaling Pathway Unveils Potential Prognostic Biomarkers for Pancreatic Cancer: A Transcriptomics Approach Article Snippet: .. The gene expression of aquaporins was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems, ThermoFisher Scientific, Waltham, MA, USA), and the following specific TaqMan pre-designed gene expression primers: AQP0 (Hs0085175_m1), AQP1 (Hs01028916_m1), AQP2 (Hs00166640_m1), AQP3 (Hs01105469_g1), AQP4 (Hs00242342_m1), AQP5 (Hs00387048_m1), AQP6 (Hs00155808_m1), AQP7 (Hs00357359_m1), AQP8 (Hs01086280_g1), AQP9 (Hs00175573_m1), AQP10 (Hs00369738_m1), AQP11 (Hs005426181_m1), and AQP12 (Hs01651303_m1) (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA). β-ACT (Hs99999903_m1) and HPRT-1 were used as housekeeping genes in cell cultures and tissues. qPCR reactions were carried out using a Article Title: SET-M33 loaded biosynthesized cellulose as effective protection against S. aureus biofilm formation Article Snippet: The concentration and purity of the RNA was then checked using a quantification spectrophotometer (DeNovix®, USA). .. The SensiFASTTM cDNA synthesis kit (meridian BIOSCIENCE) was used to synthesize cDNA from 200 ng of total RNA. qPCR reactions were performed using 2.5 μL cDNA, 12.5 μL SYBR green, 0.5 μL MgCl 2 (SensiMixTM), and 0.75 μL of forward and reverse primers ( ) at a concentration of 10 μM in clear hardshell PCR plates (Bio-Rad Inc, USA) in a Article Title: Blockage of aquaporin‐3 peroxiporin activity by organogold compounds affects melanoma cell adhesion, proliferation and migration Article Snippet: RNA quantity and quality were assessed using aNanodrop 2000c spectrophotometer and 1μg of good quality RNA (260/280 and 260/230 ratio of 2) was used to synthesize cDNA using the first-strand cDNA synthesis kit (NZYtech, Lisbon, Portugal) according to the manufacturer’s protocol. .. Real-time PCR reactions were carried out using a SYBR Green Assay:Article Title: SET-M33 loaded biosynthesized cellulose as effective protection against S. aureus biofilm formation Article Snippet: The concentration and purity of the RNA was then checked using a quantification spectrophotometer (DeNovix®, USA). .. The SensiFASTTM cDNA synthesis kit (meridian BIOSCIENCE) was used to synthesize cDNA from 200 ng of total RNA. qPCR reactions were performed using 2.5 μL cDNA, 12.5 μL SYBR green, 0.5 μL MgCl 2 (SensiMixTM), and 0.75 μL of forward and reverse primers ( ) at a concentration of 10 μM in clear hardshell PCR plates (Bio-Rad Inc, USA) in a Concentration Assay:Article Title: SET-M33 loaded biosynthesized cellulose as effective protection against S. aureus biofilm formation Article Snippet: The concentration and purity of the RNA was then checked using a quantification spectrophotometer (DeNovix®, USA). .. The SensiFASTTM cDNA synthesis kit (meridian BIOSCIENCE) was used to synthesize cDNA from 200 ng of total RNA. qPCR reactions were performed using 2.5 μL cDNA, 12.5 μL SYBR green, 0.5 μL MgCl 2 (SensiMixTM), and 0.75 μL of forward and reverse primers ( ) at a concentration of 10 μM in clear hardshell PCR plates (Bio-Rad Inc, USA) in a Control:Article Title: SET-M33 loaded biosynthesized cellulose as effective protection against S. aureus biofilm formation Article Snippet: The concentration and purity of the RNA was then checked using a quantification spectrophotometer (DeNovix®, USA). .. The SensiFASTTM cDNA synthesis kit (meridian BIOSCIENCE) was used to synthesize cDNA from 200 ng of total RNA. qPCR reactions were performed using 2.5 μL cDNA, 12.5 μL SYBR green, 0.5 μL MgCl 2 (SensiMixTM), and 0.75 μL of forward and reverse primers ( ) at a concentration of 10 μM in clear hardshell PCR plates (Bio-Rad Inc, USA) in a |